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 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
12/07/2022 |
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Actualizado : |
12/07/2022 |
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Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
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Autor : |
KALDS, P.; CRISPO, M.; TESSON, L.; ANEGÓN, I.; CHEN KEY, Y.; WANG, X.; MENCHACA, A. |
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Afiliación : |
PETER KALDS, Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.; MARTINA CRISPO, Unidad de Biotecnología en Animales de Laboratorio (UBAL), Institut Pasteur de Montevideo, Montevideo, Uruguay.; LAURENT TESSON, INSERM, Centre de Recherche en Transplantation et Immunologie, UMR 1064, Transgenesis Rat ImmunoPhenomic Facility (TRIP), Nantes, France.; IGNACIO ANEGÓN, INSERM, Centre de Recherche en Transplantation et Immunologie, UMR 1064, Nantes, France.; YULIN CHEN KEY, Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.; XIAOLONG WANG, Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.; JOSE ALEJO MENCHACA BARBEITO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay. |
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Título : |
Generation of Double-Muscled Sheep and Goats by CRISPR /Cas9-Mediated Knockout of the Myostatin Gene. |
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Complemento del título : |
Chapter 16. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Methods in Molecular Biology, 2022, Volume 2495, Pages 295-323. Doi: https://doi.org/10.1007/978-1-0716-2301-5_16 |
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ISBN : |
Online: 978-1-0716-2301-5 |
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DOI : |
10.1007/978-1-0716-2301-5_16 |
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Idioma : |
Inglés |
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Contenido : |
Abstract:
The myostatin (MSTN) gene has shown to play a critical role in the regulation of skeletal muscle mass, and the translational inhibition of this gene has shown increased muscle mass, generating what is known as ?double-muscling phenotype.? Disruption of the MSTN gene expression using the CRISPR/Cas9 genome-editing system has shown improved muscle development and growth rates in livestock species, including sheep and goats. Here, we describe procedures for the generation of MSTN knockout sheep and goats using the microinjection approach of the CRISPR/Cas9 system, including the selection of targeting sgRNAs, the construction of CRISPR/Cas9 targeting vector, the in vitro examination of system efficiency, the in vivo targeting to generate MSTN knockout founders, the genomic and phenotypic characterization of the generated offspring, and the assessment of off-target effects in gene-edited founders through targeted validation of predicted off-target sites, as well as genome-wide off-target analysis by whole-genome sequencing. Editing the MSTN gene using the CRISPR/Cas9 system might be a rapid and promising alternative to promote meat production in livestock. |
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Palabras claves : |
CRISPR/Cas9; Genome editing; Goats; Knockout; Meat production; Microinjection; MSTN; Sheep; Small ruminants. |
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Thesagro : |
CABRAS; OVEJA; PRODUCCION DE CARNE. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02176naa a2200349 a 4500
001 1063414
005 2022-07-12
008 2022 bl uuuu u00u1 u #d
024 7 $a10.1007/978-1-0716-2301-5_16$2DOI
100 1 $aKALDS, P.
245 $aGeneration of Double-Muscled Sheep and Goats by CRISPR /Cas9-Mediated Knockout of the Myostatin Gene.$h[electronic resource]
260 $c2022
520 $aAbstract: The myostatin (MSTN) gene has shown to play a critical role in the regulation of skeletal muscle mass, and the translational inhibition of this gene has shown increased muscle mass, generating what is known as ?double-muscling phenotype.? Disruption of the MSTN gene expression using the CRISPR/Cas9 genome-editing system has shown improved muscle development and growth rates in livestock species, including sheep and goats. Here, we describe procedures for the generation of MSTN knockout sheep and goats using the microinjection approach of the CRISPR/Cas9 system, including the selection of targeting sgRNAs, the construction of CRISPR/Cas9 targeting vector, the in vitro examination of system efficiency, the in vivo targeting to generate MSTN knockout founders, the genomic and phenotypic characterization of the generated offspring, and the assessment of off-target effects in gene-edited founders through targeted validation of predicted off-target sites, as well as genome-wide off-target analysis by whole-genome sequencing. Editing the MSTN gene using the CRISPR/Cas9 system might be a rapid and promising alternative to promote meat production in livestock.
650 $aCABRAS
650 $aOVEJA
650 $aPRODUCCION DE CARNE
653 $aCRISPR/Cas9
653 $aGenome editing
653 $aGoats
653 $aKnockout
653 $aMeat production
653 $aMicroinjection
653 $aMSTN
653 $aSheep
653 $aSmall ruminants
700 1 $aCRISPO, M.
700 1 $aTESSON, L.
700 1 $aANEGÓN, I.
700 1 $aCHEN KEY, Y.
700 1 $aWANG, X.
700 1 $aMENCHACA, A.
773 $tMethods in Molecular Biology, 2022, Volume 2495, Pages 295-323. Doi: https://doi.org/10.1007/978-1-0716-2301-5_16
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| 126. |  | Gimenez, A.; Rios,A. Malezas ln: Dia de Campo Cultivos de Invierno, 1989 nov 15 : La Estanzuela [Memorias]. Montevideo (Uruguay): CIAAB, 1989. p1-3 Dia de Campo Cultivos de Invierno (CIAAB)| Biblioteca(s): INIA La Estanzuela. |
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| 127. | | Gimenez, A.; Rios, A. Malezas ln: Dia de Campo Cultivos de Invierno, 1991 nov : La Estanzuela [Memorias]. Montevideo (Uruguay): INIA, 1991. p41-43 (sic) Dia de Campo Cultivos de Invierno (INIA) Participó el Proyecto Cultivos de La Estanzuela| Biblioteca(s): INIA La Estanzuela. |
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| 128. | | RIOS, A.; GIMENEZ, A. Malezas. ln: Jornada Cultivos de Verano, 1989 : La Estanzuela [Memorias]. Montevideo (Uruguay): CIAAB, 1989. p.73-78. (Resultados Experimentales ; 26).| Biblioteca(s): INIA La Estanzuela. |
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| 129. | | RÍOS, A.; FONTAINA, R. La prevención de la resistencia en Uruguay. Anexo [Preventing resistance in Uruguay.] In: INIA (Instituto Nacional de Investigación Agropecuaria); Programa Nacional Producción y Sustentabilidad Ambiental. Uso de la biodiversidad para la evaluación del impacto de la intensificación agrícola y el diseño de agroecosistemas sustentables. Las Brujas, Canelones (Uruguay): INIA, 2012. p.112 (INIA Serie Actividades de Difusión; 674) Trabajo presentado en: Pan-American Weed Resistence Conference organizada por Bayer Crop Science , Miami, Florida. 19-21 de enero de 2010.| Biblioteca(s): INIA Las Brujas. |
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| 131. | | RIOS, A.; GIMÉNEZ, A. Soja: Control de malezas In: GIMÉNEZ, A.; RESTAINO, E. (Eds.). Girasol y soja : algunos aspectos tecnológicos de producción para el litoral oeste de Uruguay. Montevideo (Uruguay): INIA, 1994. p109-114 (INIA Boletin de Divulgación; 47) Convenio INIA - Cámara de Aceites Comestibles - Facultad de Agronomía| Tipo: Capítulo en Libro Técnico-Científico |
| Biblioteca(s): INIA La Estanzuela; INIA Las Brujas; INIA Tacuarembó. |
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